RT-PCR Based Cloning and Sequencing of Potato Leaf Roll Virus-Coat Protein (PLRV-CP) Gene for Characterization as a Bangladeshi PLRV Isolate and Its Phylogenetic Analysis
نویسندگان
چکیده
منابع مشابه
Molecular Detection of Potato Leaf Roll Polerovirus through Reverse Transcription Polymerase Chain Reaction in Dormant Potato Tubers
A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...
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Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملCharacterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...
متن کاملPhylogenetic analysis of two Iranian grapevine virus A isolates using coat protein gene sequence
Symptomatic grapevine samples were collected from vineyards in Zanjan province to detect Grapevine virus A. Total RNA was extracted from symptomatic leaf samples and subjected to cDNA synthesis using random hexamer primers. Then, a DNA fragment around 800 bp including the complete coat protein (CP) gene was amplified from nine out of 57 samples by polymerase chain reaction (PCR) using specific ...
متن کاملTransformation of tobacco and potato with cDNA encoding the full-length genome of potato leafroll virus: evidence for a novel virus distribution and host effects on virus multiplication.
A full-length cDNA copy of the genome of Potato leafroll virus (PLRV) was introduced into the genome of tobacco and potato plants by Agrobacterium tumefaciens-mediated transformation. Transgenic lines were obtained in which the transgene was readily detected by PCR with DNA extracted from T(1) tobacco seedlings and clonally multiplied potato plants. PLRV-specific genomic and sub- genomic RNAs, ...
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ژورنال
عنوان ژورنال: European Journal of Agriculture and Food Sciences
سال: 2020
ISSN: 2684-1827
DOI: 10.24018/ejfood.2020.2.3.56